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ORCA-Fusion BT 数字 CMOS 相机
C15440-20UP
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ORCA®-Fusion BT 相机是科学 CMOS (sCMOS) 性能的巅峰之作。规格不折不扣：超低读出噪声、媲美 CCD 的均匀性、快速帧速率和背照式实现的高 QE。但 ORCA-Fusion BT 的优点在于，这种卓越的光子检测和采集组合能够带来什么功能。查看最暗淡的信号，从最少的光子中获取视觉上令人惊叹的高 S/N 图像，捕获以前未解决的时间事件，并自信地执行计算方法。ORCA-Fusion BT 相机使实验化繁为简，使以前不可能进行的实验变得可能。
ORCA 是滨松光子学株式会社（中国、欧盟、法国、德国、日本、英国、美国）的注册商标。
ORCA-Fusion BT 对比其他 BT CMOS 相机的优势
行业领先的独家 BT sCMOS 性能，具有值得信赖的滨松质量和可靠性
- 极低读出噪声
- 高 QE，宽波长范围
- 媲美 CCD 的均匀性；最低高读出噪声像素
- 高动态范围
- 3 种读出速度
- 光片读出模式
- 530 万像素
- USB 3.0 和高速 CoaXPress 接口
ORCA-Fusion BT 对比 EM-CCD 相机的优势
一款实用且功能强大的替代产品，可完美替代昂贵的 EM-CCD，具有出色的灵敏度、速度和通用性
- 分辨率
- 像素合并通用性
- 无放大或过剩噪声
- 高动态范围
- 大视野
用途
- 宽视野荧光显微镜
- 延时成像
- 转盘共焦显微镜
- 光片显微镜
- 超高分辨率精确定位
- 基因编码电压成像
- 光遗传学
- 单分子跟踪
- 计算成像
- 量子计算
通过低光激发进行延时活细胞成像
样品：H9c2，物镜：Apo TIRF 60XC (NA1.49)，ND：8，间隔：15 秒
细胞核
细胞核：
HCS NuclearMask 染色剂/曝光 20 ms
线粒体
线粒体：
MitoTracker/曝光 500 ms
薄膜
薄膜：
CellMask 血浆膜染色剂/曝光 500 ms
光片读出模式 [专利]
“光片读出模式”是滨松 sCMOS 相机的独特专利功能，可提高光片显微镜中的信噪比。
有关光片读出模式原理和特点的更多信息，请参阅下面的详细信息。
出版物
ORCA-Fusion BT
| Authors | Title | Source |
|---|---|---|
| Manil Bradai, Mirna Merkler, Gabriela Gil, Rebecca Davie, Shuzo Sakata | Multifiber Array-Based Photometry System for Multiregional Functional Mapping in the Mouse Brain | European Journal of Neuroscience, Volume 63, Issue 12, June 2026, e70582 |
| Michelle Küppers, W. E. Moerner | Interferometric Image Scanning Microscopy for label-free imaging at 120 nm lateral resolution inside live cells | Nature (2026). |
| Pratish Thakore, Michael G Alvarado, Sher Ali, Amreen Mughal, Paulo W Pires, Evan Yamasaki, Harry AT Pritchard, Brant E Isakson, Cam Ha T Tran, Scott Earley | Brain endothelial cell TRPA1 channels initiate neurovascular coupling | eLife 10, e63040 (2021). |
| Lexy von Diezmann and Ofer Rog | Single-Molecule Tracking of Chromatin-Associated Proteins in the C. elegans Gonad | J. Phys. Chem. B 2021, 125, 23, 6162–6170 |
| Kei Yamamoto, Haruko Miura, Motohiko Ishida, Yusuke Mii, Noriyuki Kinoshita, Shinji Takada, Naoto Ueno, Satoshi Sawai, Yohei Kondo & Kazuhiro Aoki | Optogenetic relaxation of actomyosin contractility uncovers mechanistic roles of cortical tension during cytokinesis | Nat Commun 12, 7145 (2021). |
| Mbaye Diouf, Mitchell Harling, Murat Yessenov, Layton A. Hall, Ayman F. Abouraddy, and Kimani C. Toussaint, | Space-time vector light sheets | Opt. Express 29, 37225-37233 (2021) |
| Oliver Klatt, Daniele Repetto, Johannes Brockhaus, Carsten Reissner, Abderazzaq El khallouqi, Astrid Rohlmann, Martin Heine, Markus Missler, | Endogenous β-neurexins on axons and within synapses show regulated dynamic behavior | Cell Reports, Volume 35, Issue 11, 2021, 109266, |
| Satoshi Okada, Goro Doi, Shitomi Nakagawa, Emiko Kusumoto, Takashi Ito | Simple-to-use CRISPR-SpCas9/SaCas9/AsCas12a vector series for genome editing in Saccharomyces cerevisiae | G3 Genes|Genomes|Genetics, Volume 11, Issue 12, December 2021, jkab304 |
PC 建议
随着 ORCA-Fusion BT 的推出，用户现在能够以每秒 89 帧的速度将 500 万像素的图像流式传输到他们的计算机。通过使用 ORCA-Fusion BT PC 建议中列出的指南，可以满足计算机对高数据率的建议。
软件
详细参数
| 产品型号 | C15440-20UP |
|---|---|
| 成像设备 | 科学 CMOS 图像传感器 |
| 有效像素数 | 2304 (H) × 2304 (V) |
| 像素尺寸 | 6.5 μm (H) × 6.5 μm (V) |
| 有效面积 | 14.976 mm (H) × 14.976 mm (V) |
| 最大阱容 *1 | 15 000 个电子 |
| 读出速度 | 快速扫描，全分辨率，CoaXPress：89.1 帧/秒 快速扫描，全分辨率，USB 3.0：31.6 帧/秒（16 位），42.2 帧/秒（12 位），63.3 帧/秒（8 位） 标准扫描，全分辨率，CoaXPress：23.2 帧/秒 标准扫描，全分辨率，USB 3.0：23.2 帧/秒 超静音扫描，全分辨率，CoaXPress：5.42 帧/秒 超静音扫描，全分辨率，USB 3.0：5.42 帧/秒 |
| 读出噪声 *1 | 快速扫描：1.6 个电子 (rms) / 1.5 个电子（中位数） 标准扫描：1.0 个电子 (rms) / 0.9 个电子（中位数） 超静扫描：0.7 个电子 (rms)，0.6 个电子（中位数） |
| 曝光时间 | 快速扫描：17 μs 至 10 s 标准扫描：65 μs 至 10 s 超静音扫描：280 μs 至 10 s |
| 冷却方式 | 珀耳帖制冷 |
| 冷却温度 | 强制风冷（环境温度：+25 ̊C）：−8 ˚C 水冷（水温：+25 ˚C）：−8 ˚C 水冷（最大制冷（环境温度：+20 ˚C，水温：+20 ˚C））：低于 −15 ˚C |
| 暗电流*1*3 | 1.0 个电子/像素/秒（冷却温度：-8 ˚C，环境温度：+25 ˚C） 1.0 个电子/像素/秒（冷却温度：-8 ˚C，水温：+25 ˚C） 0.7 个电子/像素/秒（冷却温度：低于 -15 ̊C，环境温度：+20 ˚C，水温：+20 ̊C） |
| 动态范围为 *1*2*4 | 21 400 : 1 (rms) / 25 000 : 1（中位数） |
| 外部触发模式 | 边缘、电平、同步读出、开始、全局复位边缘、全局复位电平、可编程 |
| 外部触发信号路由 | SMA |
| 外部触发延迟功能 | 0 μs 至 10 s（1 μs 步长） |
| 触发输出 | 全局曝光定时输出、触发就绪输出、低输出、高输出、3 个可编程定时输出 |
| 外部信号输出路由 | SMA |
| 接口 | CoaXPress（双 CXP-6）和 USB 3.0 Super Speed *5 |
| AD 转换器 | 16 bit、12 bit、8 bit |
| 透镜接口 | C 型接口（标准），F 型接口 (C15440-20UP01) |
| 电源 | AC100 V 至AC 240 V，50 Hz/60 Hz |
| 用电功耗 | 约 155 VA |
| 操作环境温度 | 0 ̊C 至 +40 ̊C |
| 存储环境温度 | -10 ℃ 到 +50 ℃ |
| 操作环境湿度 | 30% 至 80%（无雾气现象） |
| 存储环境湿度 | 90% 或更低（无雾气现象） |
*1 典型值
*2 根据最大阱容和 RMS 读出噪声的比率计算
*3 暗电流取决于冷却温度
*4 在超安静扫描模式下
*5 相当于 USB 3.1 Gen 1 (SuperSpeed USB 5 Gbps)
光谱灵敏度特性
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